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KMID : 0379119910190030214
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Korean Journal of Mycology
1991 Volume.19 No. 3 p.214 ~ p.219
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Studies on the Adenosinetriphosphatase in the Mushroom (1)
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Abstract
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Adenosine-5´-triphosphatase(F₁-ATPase) in the Lentinus edodes was fractionated by ammonium sulfate 30% saturation and purified by Sephadex G-200 gel filtration in three times. Three kinds of protein fractions of F₁-ATPase were isolated from this mushroom, and fraction I and II showed its activity for the substrate, adenosine-5´-triphosphate. Its optimum pH and temperature were found to be pH 7.6 and 58℃, and its thermal stability was stabled for 30 min. at 20-30℃. The Km value of this enzyme was 1.81 mM.
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